Extraction of arecoline from Areca inflorescence at different blossoming stage by heat reflux. With yield of arecoline as index, the samples were determined by HPLC. The result showed that the yield of arecoline was 4.96±0.067 mg/g，4.26±0.14 mg/g and 2.02±0.16 mg/g in beginning flower, open flower and ending flower respectively. The result indicated that the arecoline content was coming down with the Areca inflorescence was becoming mature at different flavoring period. It will provide the reference for the harvesting and processing of Areca inflorescence. Key words: Areca inflorescence; Areca inflorescence tea; Areca inflorescence wine; arecoline; HPLC