The substrate DL-5-hydroxymethylhydation was synthesized in a chemical process from DL-serine by amino acid cyclization method with the yield of 93.5%. And then D-serine was produced by the biotransformation of DL-5-hydroxymethylhydation under the condition of pH 9.0,temperature of 38℃ and the conversion time for 42 hours with the yield of 81.7%.