Due to its sustained secondary structure and plenty of thiols, Keratin obtained via reduction system could be used to prepare Protein-polymer hybrids(PPHs) bio-materials. In the paper, pig hair was dissolved in reduction mixture under optimized conditions (pH 6.5, NaS2O5 1.0 mol/L, Urea 14 mol/L, SDS 0.2mol/L, 8h, 65℃) to extract keratin, it gave conversion of 55-60%. The FT-IR spectra and TGA curves of result keratin, when compared with the original hair, showed that its secondary structure was maintained. Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis indicated that the keratin had molecular weight distribution of three bands (13kDa-15kDa、45kDa-50kDa and 52kDa-55kDa). The result of Ellman test indicated that free thiols of keratin covered about 38.9% of total sulfur in keratin.