α-Amino dodecanoic acid (ADA) was synthesized by the amination of α-chloro dodecanoic acid (CDA) derived from chlorination of natural dodecanoic acid, and the molecular structure of ADA was identified by FT-IR、ESI-MS and 1H-NMR. Effects of reaction temperatures, reaction times, dosages of catalyst and ratio of raw materials on the yield of ADA was investigated, unfortunately, the yield of ADA was lower in normal temperature technology which was accounted as the competition of the side hydrolysis reaction. A temperature programming of normal temperature followed by hydrothermal was applied to inhibit hydrolysis effectively, which evolved the amination a practical organic synthesis reaction. The yield of ADA was significantly improved from about 80% under the normal temperature technology to 91% under the optimal temperature programming technology when the amination was run with n(ammonia) :n(CDA) = 20 :1 at 70 ℃ for 12 h following 100 ℃ for 4 h.