Column chromatographic technique was used for the isolation and purification of the chemical constituents of the n-butanol portion and the petroleum ether portion from Elatostema duyunens.,18 compounds are separated, and 10 compounds were identified by MS and NMR data analysis, Quereetin(1), Quercetin-3-O-β-D-galactoside(2), Quercetin-3-O-β-D-glucoside(3), Kaempferol(4), Kaempferol 3-O-β-D-glucoside(5), Oleanic acid(6), β-sitosterol(7), Ursolic acid(8), stigmasterol(9), and n-pentacosane(10), respectively.