Skeleton isomerization of n-butene over ZSM-35 Zeolite with high Si/Al ratio on a fixed-bed reactor was investigated. The used catalyst was regenerated by high temperature oxidation method. Information about the effect of carbon deposition was obtained by TPO、BET、NH3-TPD and Py-IR measurements.. The results indicated that the catalyst exhibits good stability and regenerability, furthermore, the conversion of butene was 45% and the selectivity of iso-butene was 95%, when increasing TOS to 390h, the yield of iso-butene was still maintained above 32%. Deposited carbon was substantially formed during the initial period in micropore preferentially on strong acid sites and was mainly graphite carbon which was difficult to be removed.