In this paper, sebaconirile had been successfully prepared using sebacic and ammonia as raw materials under the condition of catalysts. The effects of catalysts with different compositions, reaction temperature and time were studied. The results showed that the optimal synthesis condition as follows: the lower temperature region (140-210 oC) were favorable for amidition reaction and the corresponding reaction time is 8h, however the dehydration reaction is very effective under the higher temperature region (210-265 oC) and the corresponding dehydration time is 5h. ZnO/Al2O3 catalysis with mass faction of 6% revealed the yield of 83%. The crude product had been characterized by HNMR and the purity of prepared sebaconitrile was 92% via temperament chromatograph , which indicated that the sebaconitrile synthesized in this paper would have the good value of industrialization