The characteristics of adsorption of Cr3+ on the collagen fiber(CF) were studied with chromium solution as target; The effects of temperature, time, pH, adsorbent dosage, initial concentration and salt ion concentration (Na+, Ca2+) on the adsorption process were reseached through the single factor experiments, respectively. The results indicated that the collagen fibers have strong adsorption abilities to Cr3+ and the adsorption capacity can be up to 90mg/g, the optimal pH and temperature of adsorption were 4.5 and 40℃, respectively; For a certain amount of chromium solution, the adsorption capacity was increased with the increasing of initial concentration of Cr3+, but decreased along with the increasing of adsorbent dosage. With the extension of time, the adsorption capacity increased gradually and finally reached adsorption equilibrium. The salt ions in the solution will have inhibitory effect on the adsorption process, and the inhibition enhanced with the increasing of ion concentration. The adsorption thermodynamics and adsorption dynamics were studied, by comparison, it was found that the Freundlich equation and Pseudosecond-order kinetic model can better describe adsorption behavior of Cr3+ on the collagen fiber.