3%MoO3/γ-Al2O3 catalysts with different Re2O7 contents were prepared via conventional incipient-wetness impregnation method. Mixed butene self-metathesis reactions over these catalysts were investigated in a fixed-bed reactor, where mixed butene (n(1-butene)∶n(2-butene)=3) was chosen as reactant. The catalysts were characterized by means of XRD，NH3-TPD，H2-TPR and N2-adsorption. The results indicate that addition of rhenium in 3%MoO3/γ-Al2O3 catalyst increased the acid amount, inhibited the induction period in the initial stage of the reaction, enhanced the activity and stability of catalysts in mixed butene self-metathesis reaction. Under the reaction conditions of 80 ℃, 1.0 MPa, WHSV=1.2 h-1, 1%Re2O7-3%MoO3/γ-Al2O3 catalyst gave butene conversion above 60%, propene yield remained at about 25% during 70 h on stream.