Abstract: Acetobacter xylinum strain was screened in our own laboratory,Using coconut water as incubating system,Bacterial cellulose (BC) were prepared by Static fermentation culture method using Acetobacter xylinum. the structure and properties of the BC and commercial BC were tested and compared. Observe the microstructure characteristics of BC. Results showed Preparation of BC and commercial BC are similar in structure and some properties，They are colorless, transparent film and a three dimensional network structure and pores structure, It proves that BC has fine nano fibre-network, it has good moisture absorption and excellent holding water capacity. The BC production cost is low，And according to need a lot of preparation, which has extensive usage in biomedicine areas.