On the basis of deterrmined the pigment content analytical method for anthraquinones pigment content, an Orthogonal test L9(34) was used to observed the optimal extraction process of ethanol extract anthraquinones pigments from walnut green husks, and the effects of the pigment stability on oxidants, reducing agents, light, metal ions were also investigated. The results showed that this analysis method has high precision and accuracy (recovery is 90.44% ~ 103.52%), with well stability and good reproducibility. The linearity with correlation coefficient 0.9997 was found within the range of 0.00416 mg/ml to 0.0250 mg/ml. The highest pigment extraction rate can be obtained when solid-liquid ratio is 1:30(g．ml), ultrasonic time is 30min, extraction temperature is 60℃ and ethanol concentration is 50%. Research results for pigment stability also displayed that the metallic ions K 、Na 、Mg2 、Ca2 and Zn2 have no apparent effect, and Fe3 has hyperchromic effect significantly.