In search of effective antitumor agents, According to substructure link principle, The quinolone compounds and α-hydroxyphosphonates were jogged. Eight O,O' - dialkyl - α - phenyl - α - substituted quinolinylanoxy methylphosphonate derivatives were designed and synthesized. The structures of all the compounds were confirmed by IR, NMR and elemental analysis. The bioassay of target compounds were tested. The result indicated that they exhibited certain antitumor activities. And compounds 3c, 3g showed obvious inhibition effect on SGC-7901 cells growth in vitro at 20 μ mol/L, inhibition ratio reached 50.7 %, 46.4 %, respectively.