Abstract:The β-cyclodextrin inclusion compound of 2-Mercaptopropionic acid was prepared by saturated water solution method,the content of 2-Mercaptopropionic acid of inclusion compound were tested by ultraviolet spectrophotometry and the synthesis process was optimized by orthogonal experimental design ,the results show that the most optimized conditions were as following:β-cyclodextrin:2- Mercaptopropionic acid =12:1 (Mass ratio); the inclusion temperature was 45 ℃; the inclusion time was 4h; the inclusion rate can reach 74.95%;then the structure of the inclusion complex was characterized by digital liquid crystal biological microscope, scanning electron microscope, ultraviolet spectrophotometry and infrared spectroscopy.