A series of pyrazoline derivatives were designed and synthesized from acetylferrocene, benzaldehyde, hydrazine hydrate and acyl chloride. The structures of the target compounds were confirmed by 1HNMR, FTIR and ESI-HRMS. Finally，the primary antimycotic activity of the title compounds against four plant fungals (Gibberlla saubinetii, Pythium solani, Gibberlla nicotiancola, Fusarium oxysporium f.s.p. niveum.) had been screened. The final results indicate that some of the compounds have a good plant anti-fungal activity.