A series of catalysts made of tungsten oxide loaded on SiO2, γ-Al2O3, and SiO2-Al2O3 were tested for esterification of acetic acid with ethanol. The catalysts were studied by characterization of XRD, nitrogen adsorption measurement, Raman and NH3-TPD in detail. The results indicated that tungsten oxide supported on SiO2-Al2O3 lead to the stronger and more acid sites, which appeared to be more effective for esterification of acetic acid with ethanol. Among these catalysts, SiO2-Al2O3 supported 8.0 wt% tungsten catalyst gave the highest activity for esterification of acetic acid with ethanol reaction with the conversion of acetic acid up to 69.7 %. Bulky crystalline WO3 loaded on SiO2-Al2O3 was disadvantage for improving activity of esterification.