Cu(Ⅱ), Ni(Ⅱ), Zn(Ⅱ), Pb(Ⅱ), Cd(Ⅱ) complexes with Schiff base derived from diacetylferrocene and hydrazine hydrate have been prepared. The ligand and its complexed have been characterized by molar conductance, elemental analysis, IR spectra and 1H NMR. All compounds were screened by antibacterial activity. The final results indicate that all compounds had inhibitory activity. Metal complexes 2a–2f had moderate to excellent antimicrobial activity in comparison with the ligand compound. Among these compounds, CdL(CH3COO)2(2f) had the best inhibitory activity.