Amphiphilic derivative of chitosan oligosaccharide (COS), N-linoleyl chitosan oligosaccharide (LCOS), was obtained by introducing linoleic acid into COS through acylation reaction. The chemical structure of LCOS was characterized by IR, elemental analysis and Gel permeation chromatography (GPC). The micellization behavior of LCOS in aqueous solution was studied by fluorescence spectroscopy using pyrene as a probe, and the meso-tetraphenylporphyrin-loaded LCOS micelles (TPP-LCOS) were prepared. The thermodynamical parameters of TPP-loaded process were determined by isothermal titration calorimetry (ITC). The results showed that the substitution degree of the product was about 42.1%. The critical micelle concentration of LCOS was 2.00×10-3 g/L. TPP could be efficiently loaded by LCOS into spherical nano-micelles, and the particle size of the TPP-loaded LCOS micelles was between 60 and 100 nm. In addition, the medium-strength combination between LCOS micelles and TPP (Ka=2.87×106 L/mol) would be expected to slow release of TPP and enhance the efficacy time.