Ni/SiO2 catalysts modified by Al, Cr, Cu and Zn were prepared by precipitation and applied in fixed-bed gas-phase catalytic cyclization of ethanediamine (EDA) with glycerol to 2-methylpyrazine (2-MP) in the present work. And at the same time, the effects of calcination and reaction temperature, GHSV and water content on the catalytic properties of Ni-Zn/SiO2 in cyclization were also investigated. The results indicated that the presence of ZnO led to the simultaneous production of acidic and alkali sites over Ni-Zn/SiO2, which was helpful to the activation of EDA and glycerol molecules and 2-MP formation through cyclization was improved. It was also found that the calcination temperature was an important factor on affecting the catalytic property of catalysts in cyclization. And Ni-Zn/SiO2 calcined at 500 oC exhibited the highest 2-MP yield of 66.4% along with complete conversion towards glycerol and EDA was obtained over Ni-Zn/SiO2 catalyst under the optimum reaction conditions: reaction conditions 380 oC, GHSV of carrier N2 150 h−1, water content in fed reaction liquid 70%.