2-Amino-6-chloropurine as the starting material was treated with 2-bromoethyl acetate to product 9-acetoxyethyl-2-amino-6-chloropurine (Ⅰ, 65.6% yield). Compound Ⅰwas diazotized and reacted with disulfides to afford 9-acetoxyethyl-2-alkythio-6-chloropurine (Ⅱ, 58.3-72.1% yield). NaN3 was reacted with Ⅱto acquire 9-acetoxyethyl-2-alkythio-6-triazo purine (Ⅲ, 86.4-92.0% yield). The result of NMR test (Ⅲ)showed that there were azido tautomer(A)and tetrazolyl tautomer(T) in the solution, and the ratio of azido tautomer was 61.5-67.2%. We use PPh3-HCl-DMSO as the reaction solvent to reduced trizao to amino and hydrolyzed the ester completely to afford the 2-alkylthio-6-amino-9- (2-hydroxylethyl)purines (Ⅳ) in the good yield（~90%） in one pot. All the compounds synthesized were comfirmed through IR, 1H NMR, 13C NMR, and HRMS.