Because of low cost and high yield of fermentable sugars, acid-based pretreatment methods have been proven to be efficient in conversion of lignocelluloses to fuel ethanol by microbial fermentation. However, some of the byproducts including furfural and 5-hydroxymethyl furfural（5-HMF) in the acidic prehydrolysates were the inhibitors in the fermentation process. Meanwhile, large amounts of enzymatic hydrolysis lignin (EHL) residue were produced as another byproduct. To explore the application of this lignin residue for detoxification of acidic prehydrolysates, EHL was applied to decrease the concentration of furfural and 5-HMF through adsorption. The effects of contact time, lignin dose, pH on the adsorption of furfural and 5-HMF on EHL and adsorption isotherms were investigated. EHL was used to remove furfural and 5-HMF in two acidic prehydrolysates of corn stover. The results showed that the adsorption was a spontaneous exothermic process, and good fit was obtained by Freundlich model. Furfural was easier to be removed than 5-HMF through adsorption by EHL. The results indicated EHL could be the adsorbent to remove furfural and 5-HMF in acidic prehydrolysates.