The low cost naturally immobilized lipase CPL from the Carica papaya was used to catalyze kinetic resolution of aromatic secondary alcohols. Taking the 4-phenyl-2-butanol as the model substrate, the effects of reaction temperature, reaction solvent, acyl donor and substrate molar ratio on the resolution ability of CPL were investigated. The results showed that the optimum reaction conditions were as follows: reaction temperature of 30~45℃, acyl donor of active vinyl ester, n-hexane or cyclohexane as reaction solvent and substrate/acyl donor molar ratio of 1:2. Under these conditions, the substrate conversion was close to 50%, e.es was more than 99%, and enantiomeric ratio E was higher than 200. CPL also showed good resolution ability to other similar structure aromatic secondary alcohols. This work provides a new biocatalyst alternative for economic preparation of chiral aromatic secondary alcohols.