Abstract: Poly(amine-ester)-1,8-naphthalimide dendrimer (PAE-1,8-NL) is synthesized from ethylenediamine (EDA), trimethylolpropane triacrylate (TMPTA) and 1,8-naphthalic anhydride. In the energy transfer systems of PAE-1,8-NL and dyes (Eosin B, Rhodamine B, Titan yellow , Methylene blue and Methyl orange) , The changes of fluorescence intensity were studied. The results show that the fluorescence of dyes increased when PAE-1,8-NL added the dyes DMF solutions. The fluorescence of dyes is increased and then decreased by PAE-1,8-NL. The rank of their fluorescence enhancement ability was Methylene blue > Rhodamine B > Methyl orange > Eosin B > Titan yellow. In DMF, acetone and acetonitrile solvent respectively, the different fluorescence intensity of the effect of PAE-1,8-NL on dyes.