Abstract：The ordered mesoporous materials V/SBA-15 was prepared by hydro-thermal method under the acidic conditions using P123(tri-block copolymer) as template, tetraethoxysilane(TEOS) as silicon source, citric acid vanadyl complexes as precursors. The influence factors of preparation mesoporous molecular sieves V/SBA-15 including the different crystallization time and the dosage of template agent were systematically investigated. The experimental results showed that under the optimum condition such as the crystallization time 96h and dosage of template agent n=1.0, V/SBA-15 remains a highly ordered two-dimensional hexagonal pore structure of SBA-15, and the load of active ingredient metal vanadium oxide uniformly distributed on the surface of mesoporous. The structure of V/SBA-15 was characterized by the XRD, BET, FT-IR, meanwhile, the catalytic performances were verified by the selectivity and yield of acetophenone in the oxidation of ethyl-benzene to acetophenone reaction. The results displayed under the optimum condition of catalyzation oxidation ethyl-benzene to acetophenone, the selectivity and yield of acetophenone are 82.65% and 38.96%, respectively.