Poly(DEGMA-co-OVNG) with narrow polydispersity (PDI) and obvious thermosensitivity was effectively prepared via enzymatic synthesis combined with Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization. The structure and molecular weight of the synthesized glycopolymer were characterized by 1H NMR, FTIR and gel permeation chromatography (GPC), respectively. Measurement by Dynamic Light Scattering (DLS) and transmission electron microscopy (TEM) revealed that Poly(DEGMA-co-OVNG) was able to self-assemble into nano-micelles with well-defined structure and uniform size in aqueous solution. UV-visible spectroscopy tests showed that the low critical solution temperature (LSCT) of the polymer can be controlled by the molar ratio of monomers. Especially, as the molar ratio of DEGMA and OVNG was 8:1, the LCST of Poly(DEGMA-co-OVNG) could be 36oC in aqueous solution. The results demonstrate that Poly(DEGMA-co-OVNG) can form nano-micelles in the human body environments, which may have potential as functional drug carriers.