A series of NiWB-M/CNTs-A catalysts were prepared by ultrasonic impregnation chemical reduction method for the conversion of microcrystalline cellulose. The catalysts were characterized by means of NH3-TPD and BET. The influence of different ratio of Ni/W, loadings of NiWB in the catalyst and the reaction conditions were discussed. The BET results showed that the conversion of microcrystalline cellulose is directly related to the pore diameter of the catalyst instead of specific area of the catalyst .The NH3-TPD results showed that Strong acid centers of the catalyst play a critical role on the conversion of microcrystalline cellulose .The conversion of microcrystalline cellulose was up to 100%, the yield of ethylene glycol was 57.66% and the yield of hexitols was about 10% under the optimum conditions of 250 ℃, H2 pressure 6.0 MPa for 2.0 h.