Abstract: The bioleaching of vanadium from vanadium-bearing stone coal has been studied by using Acidthiobacillus ferrooxidans , in order to determine the feasibility and optimal technological conditions. The influence of parameters such as pulp density, leaching temperature, initial pH and oscillation speed on vanadium leaching efficiency was investigated. On the basis of single-factor experiments, the conditions including leaching temperature , initial pH and oscillation speed for the bioleaching were optimized by using Box–Behnken Design (BBD) and Response Surface Methodology (RSM). The results showed that the leaching efficiency of vanadium could reach 69.2%, when the optimum condition of the experiment was 3% for pulp density, 32 ℃ for leaching temperature, initial pH value of 2.1 and oscillation speed of 147 r/min．