The magnetic microspheres with pH- and thermo-sensitivity were prepared by dispersion polymerization in the presence of Fe3O4 magnetic fluid in an ethanol/water medium, using styrene, methacrylic acid, N,N-methylene-bis-acrylamide and N-isopropylacrylamide as comonomers. The morphology of the magnetic microspheres was studied by SEM. The diameter of microspheres was 1.5μm. The adsorption kinetics of bovine serum albumin were investigated. The equilibrium adsorption capacity was 565.6mmol/g. The adsorption kinetic was controlled by liquid film diffusion. The adsorption process could be described by Freundlich model.