The Self-emulsified latex and external emulsified latex have been prepared by acrylic (acrylate) monomer grafted chromium-containing protein which extracted from tannery solid wastes, and then used them filling to wet-chromed bovine hide. The stability of two kinds of latexes were analyzed by Zeta potential analyzer and high-velocity centrifuge, the results indicated that external emulsified latex had a better stability than self-emulsified latex. Ninhydrin color reaction and FTIR spectra proved the existence of acrylate polymer grafted protein polypeptide. Self-emulsified latex’s percent grafting (PG) and grafting efficiency (GE) were 94.37 % and 56.62 % and external emulsified latex’s were 113.05 % and 67.83 %, respectively. Application research found that latex had some obvious retanning characteristics to wet-chromed bovine hide, and self-emulsified latex’s thickening effect better than external emulsified latex’s.