This paper presents an efficient synthesis of tulobuterol-D9 with self-made tert-butylamine-D9 as the labeled precursor. Tulobuterol-D9 was synthesized from O-chloroacetophenone as the starting material by bromination with CuBr2, amination with tert-butylamine-D9, reduction with NaBH4. Uniform design method was used to study the effects of the process conditions and experiment data was analyzed by quadratic polynomial regression. The optimized reaction parameters were: reaction temperature is 59 ℃, reaction time is 5 h, dosage of solvent is 25 mL, the molar ratio for bromoacetophenone and t-butylamine-D9 is 1:2. As-synthesized product was confirmed by MS, NMR and HPLC to be target compound. Its chemical purities is higher than 98.0 % and isotopic enrichment is higher than 97.5 %（atom D）. The above compound could be used as internal standard in the field of food safety.