The properties and phase behavior of different glycoside-based surfactant/ didecyldimethylammonium Chloride (DDAC) mixtures have been investigated. The three glycoside-based surfactants are decyl glycoside (APG), dodecyl ethoxy glycoside (AEG) and disodium dodecyl ethoxy glycoside citrate (AEG-EC). For APG/DDAC, synergism was observed in surface tension, foaming and emulsifying properties, not in wetting ability. For AEG/DDAC, synergism was observed in foaming and emulsifying properties, while the wetting ability exhibited no such effect. The surface tension and wetting ability of AEG-EC/DDAC mixed systems showed synergism, while the foaming property not. Phase behavior of the glycoside-based surfactant/DDAC/water ternary system has also been carried out by polarized optical microscope. The phase diagrams are characterized by a micellar phase, a region of coexistence of lamellar and micellar phases and a lamellar phase.