A series of anionic stable waterborne polyurethanes(WPUs) based on hydroxyl-terminated poly(butadiene acrylonitrile) (HTBN) as the soft segments were synthesized ，and the effect of different content of HTBN on the properties of WPU were discussed. Fourier transform infrared (FT-IR) was used to characterize the structure of the HTBN and WPU; Particle size, DSC, XRD, stretching and TG tests showed that with the increasing of the content of HTBN, the average particle size of WPUs increased gradually, the tensile strength increased first but decreased then，the heat resistance significantly improved. When the content of HTBN was 40%, the tensile strength was the highest, catching 20.2 MPa, the elongation at break was 521%.