Emulsion liquid membrane system containing mobile carrier is widely applied in separation and preconcentration of trace substances in wastewater. The polyamine surfactants such as polyisobutylene-bis-succinimide (T-154) have attracted considerable interest due to good emulsifying and dispersing properties. In the paper, the surfactant was used as double effects of emulsifier and mobile carrier in the emulsion liquid system for the first time. The enrichment performance for phenol was investigated by the emulsion liquid membrane. Established stable conditions of the emulsion liquid membrane are as follows: the volume ratio of T-154 to liquid paraffin to kerosene is 4:2:30, the mass concentration of sodium hydroxide (NaOH) solution used as the internal phase is 3%, the ratio of oil phase to internal phase is 1:2. The separation conditions involved the pH value of feed phase (pH=6), the ratio of emulsion to external phase (1:4), and enrichment time (15min) were founded. The phenol in the wastewater was separated and enriched with the prepared emulsion. The satisfactory results show that the recovery of standard addition is between 96% and 98%, the relative standard deviation is between 3.7% and 4.8%. The established method has the advantages of simple operation, low capital and operating costs and high efficiency, which can be used to remove phenol from aqueous media such as wastewater.