PolyoxyethylenePolyoxyethylene-polyoxypropylene-polyoxyethylene (P123) was used as a template and 1,3,5-trimethylbenzene (TMB) was used as a swelling agent. A mesoporous material was synthesized using 1,2-bis(triethoxysilyl)ethane(hexaethoxydisilethylene) (BETS) and (3-cyanopropyl)triethoxysilane (CTES), and α-glucosidase was successfully immobilized on this material. The effects of 5 organic solvents on the immobilized enzyme were then investigated. Over 50 h, the following residualstable residual enzyme activities were observed: 90% in 25% ethanol, 45% in 25% methanol, 40% in 25% isopropanol, 25% in 25% acetone and 42% in 10% acetonitrile. Although there are certain limitations for α-glucosidase immobilized on mesoporous materials in organic solvents, some activity remained. Therefore, mesoporous materials are expected to be utilized to immobilize α-glucosidase in subsequent research and industrial applications.