This study indicates the design and investigation of two continuous reaction processes for efficient production of D-lysine including an enzymatic racemization step and an enzymatic decarboxylation step. Firstly, L-lysine was racemized to the DL-lysine by whole cells containing amino acid racemase (AAR) ; then eliminating AAR and adding whole cells containing lysine decarboxylase (LDC), L-lysine in the mixture was selectively decarboxylated to 1, 5-pentanediamine and CO2; finally D-lysine was separated from mixture by isoelectric point crystallization and ion exchange resin. An optimal condition of the cascaded enzyme system is as follows: reaction temperature 40 oC, 0.2 mol/L of potassium phosphate buffer (pH=5.8), 50 g/L of L-lysine, 10 g/L whole cells of AAR and LDC, respectively, 4 mmol/L of PLP, 0.1 g/L of Triton-X 100. Under the optimal conditions, after 12 h including 2 h of racemization step and 10 h of decarboxylation step the enzymatic catalysis reaction was completed, and the yield of D-lysine could reach 42%, enantiomeric excess (e.e.) =98%.