Glu-Co/Mn-CCTS was synthesized via the combination of amino group of glutamic acid with imprinted crosslinked chitosan (Co/Mn-CCTS) by combining sol-gel method with reverse phase suspension method involving both manganese nitrate and cobalt nitrate as imprint molecules. Their structures were analyzed utilizing the techniques of FTIR and SEM. The static adsorption experiments for Co2 and Mn2 with low concentration indicate that the initial pH, temperature and initial Co2 and Mn2 concentrations, contact time, presence of other cations have great effects on the adsorption performance. The adsorption datas fit to pseudo-second-order , Langmuir and Freundlich models for both Co2 and Mn2 . The removal rates of Co2 and Mn2 are all above 98% at pH values from 4 to7 at 303K, which reach 96% after 2 h at pH value of about 5.5. The adsorption performance of Co2 on Glu-Co/Mn-CCTS compared favorably with that of Mn2 in presence of other cations.