A serious of yCuO/CuxCe1-xOδ catalysts were prepared by combinations of citric acid sol-gel method and impregnation method. The influences of CuO content in support (the x) and CuO loading (the y) on the catalytic performance in CO oxidation were characterized by XRD, BET and H2-TPR techniques. The results showed that the copper entering into the cerium oxide crystal lattice could act synergistically to promote the dispersition of copper on support, reducibility and catalytic activity. The orthogonal experimental design was used to optimize the CuO contents and relevant data was processed by the DPS (Data Process System) software. The results indicated that the 16.40%(w)CuO/Cu0.05Ce0.95Oδ catalyst exhibited the best catalytic performance, and under the conditions of 50 ℃ and 3.0 MPa, it could remove the CO in liquid propylene from 1.0 × 10-5(φ) to 3.0 × 10-8(φ) with good stability of 1400 min.