The single-walled carbon nanotubes (SWNTs) were oxidized by sulfuric acid and nitric acid to prepare carboxylated single-walled carbon nanotubes(SWNTs-COOH), and then dispersed with a amphiphilic Pluronic F127((PEO)99(PPO)65(PEO)99) triblock copolymer under the condition of magnetic stirring,shock and ultrasound to prepare the suspension of SWNTs-COOH/F127.The dispersion and stability were carried by static observation.The characterization of structure of modified SWNTs was carried out by laser particle size analyzer,scanning electron microscope,transmission electron microscopy,fourier-transform infrared spectra,UV–vis spectroscopy and Raman spectroscopy.The dispersion of SWNTs、F127 modified SWNTs、carboxylated SWNTs、carboxylated SWNTs modified by F127 was compared and the experimental results showed that after mixed acid treatment,the single-walled carbon nanotube have modified with carboxyl groups successfully.Compared with the pristine SWNTs,the dispersion and stability in water of modified SWNTs have greatly improved.The SWNTs-COOH/F127 suspension has no deposition after centrifugation for 30min with 4000r/min and can stand at room temperature for more than 30 days without flocculation.The aqueous SWNTs-COOH/F127 of high dispersibility was attempt to construct a rapid detection system.