Various quaternary ammonium salts with formate (DDAF), acetate (DDAA), propionate (DDAP), ethacetate (DDAE), chloridion (DDAC) and nitrate (DDAN) being counter ions were researched. The surface activities and potential application were measured. We find it is interesting to notice that DDAF, DDAA, DDAP and DDAE have the higher value of the critical micelle concentration and the minimum surface tension than traditional surfactant DDAC, and DDAN shows higher surface activities than traditional surfactant DDAC. DDAF, DDAA, DDAP and DDAE show excellent emulsifying performance and wetting power ability, while its foaming power is low. In addition DDAN has higher foaming power, emulsifying performance and wetting power than DDAC. Moreover, the bacteriostatic tests show that DDAF, DDAA, DDAP, DDAE and DDAN have greater efficacy than DDAC against E. coli and S. aureus.