The molecularly imprinted monolithic column(MIMC) was synthesized using α-methacrylic acid as functional monomers, ethylene glycol dimethacrylate as cross-linker, the mixture of isopropanol and dodecadol as porogenic reagent and Ginsenoside Re as template molecules by in-situ polymerization. The performances of separation and adsorption of MIMC were investigated, the results of research indicated that optimal mobile phase was methanol and water (40:60, v/v), separation factor 1.769, the adsorption isotherm followed the anti-langmiur isotherm model by frontal chromatography, saturated adsorption capacity was 0.247mg/g. MIMC has special molecule recognition ability to Re. The result of scanning electron microscopy(SEM) showed that the unique porous structure was formed in the polymer, which was beneficial to the flow of mobile phase and the mass transfer of template molecules.