In view of high temperature and salt of carbonate reservoirs, a novel temperature-resistant and salt-tolerant surfactant BHJ-2 was synthesized. The temperature- resistance and salt-tolerance performance, interfacial activity, ability of changing wettability, adsorption property, washing oil property and displacement efficiency were evaluated. The results showed that BHJ-2 has capacity of temperature-resistant 140°C and salt-tolerant 2.33×105 mg/L, respectively, reduce adsorption loss to 0.574 mg/g, change oil-formation water-rock contact angle from 109.6° to 61.4°. After aging for 30 days under temperature of 140°C, the oil-water interfacial tension and washing oil efficiency of BHJ-2 were almost unchanged, interfacial tension between BHJ-2 and oil was 0.576 mN/m, washing oil efficiency under static condition was 83.7%, enhancing the displacement efficiency by 36.7%. BHJ-2 has a promising application in high temperature and salinity carbonate reservoirs with lipophilicity.