[Objective] This paper studied the preparation, properties and specially antibacterial ability of n-HA/PVA/CS composite hydrogel which aims to provide theory evidence to support its further uses in artificial cornea scaffold materials. [Methods] Physical cross-linking was adopted to prepare composite hydrogel. Then composite hydrogel was studied by testing its moisture content, mechanical properties and micro structure. Turbidimetric and plate counting method were adopted to do researches about the minimum inhibitory concentration and the inhibitory rate of n-HA/PVA/CS after early exploration. At the same time, the different inhibitory rate between Gram-positive bacteria S.aureus and Gram-negative bacteria E.coli were be analyzed. [Results] n-HA/PVA/CS composite hydrogel was evenly spread three-dimensional porous structure, moisture content was 75%, the tensile strength was 0.26 MPa. n-HA/PVA/CS composite hydrogel had strong antibacterial ability after disposed by 2% acetic acid.The MIC of n-HA/PVA/CS on S.aureus cells and E.coli cells both were 0.5g/L. When the concentration of n-HA/PVA/CS was 2.0g/L, the inhibitory rate of S.aureus and E.coli reached 84% and 99%, respectively. When the concentration of n-HA/PVA/CS was 2.5g/L, the inhibitory rate reached 100%.Therefore, n - HA/PVA/CS composite hydrogel was expected to become the artificial cornea scaffold material with excellent performance.