Abstract: The aim of this research was to prepare the CoQ10-loaded nanostructured lipid carriers by high pressure homogenization method. The optimized CoQ10-NLC was assessed for particle size、polydispersity index、Zeta potential、retention rate and in vitro permeation in pig skin. The results showed that CoQ10-NLC had good dispersing and high physical stability. Especially, after a month the retention rate of CoQ10 was higher than 80% at the storage condition of 4 ℃ . In vitro transdermal study indicated CoQ10-NLC enhanced the permeation of CoQ10 in the viable epidermis and dermis.