A series of catalysts, including monometallic catalyst Ti-SBA-15-36, Mo-SBA-15-(180/28) and bimetallic catalyst Mo-Ti-SBA-15-(X), were synthesized by the one-step method. The influence of Mo content in the bimetallic Mo-Ti-SBA-15-(X) on catalyst structure and catalytic property was investigated. The reasons for the differences’ catalytic activity in the epoxidation cyclohexene and their synergy mechanism were analyzed. The reaction conditions in the epoxidation cyclohexene reaction were studied. The results showed that the optimum bimetallic catalyst Mo-Ti-SBA-15-(180/28) more catalytic activities than the monometallic catalyst Ti-SBA-15-36, Mo-SBA-15-(180/28). When the reaction condition (catalyst amount: 25mg, cyclohexene: 2.5mmol, TBHP: 5mmol, 1,2-DCE: 10mL, reaction temperature: 80oC, reaction time: 8h) was used, the conversion of Ti-SBA-15-36, Mo-SBA-15-(180/28) and Mo-Ti-SBA-15-(180/28) was 41.07%, 46.44%, 98.33%, respectively, and the selectivity was 97.56%, 93.19%, 98.86%, correspondingly. And the catalyst Mo-Ti-SBA-15-(180/28) has a good reusability.