Abstracts: This paper built a three-phases system composing of the feed phase, the membrane phase and the stripping phase using supported liquid membrane technique, which made the separation of lincomycin from aqueous solutions come true. The system of lincomycin+isoparaffin(Isopar L) in kerosene+HCl was used. The effects of initial lincomycin concentration (Cb f) in the feed phase, the pH of the feed phase, the pH of the stripping phase and the extraction solvent volume fraction (VF) on the distribution coefficient (D) were studied. Besides, we optimized the operating conditions of membrane module to realize the extraction. We also established a mathematical model to analysis the mass transfer process and the resistance of the process. The results show that the maximum distribution coefficient D=2.34 can be obtained with 11.9 mmol/L of lincomycin, 80% Isopar L(volume fraction) in the extractant, pH=10.1 in the feed phase and pH=1.2 in the stripping phase. The optimum operating conditions of membrane module is 520:500 (mL/min) of tube side flow rate Vf and shell side flow rate Vs. Mass transfer resistances were analyzed, the tube-side resistance is 6.7×105s/m, the shell-side resistance is 3.7×105s/m and the membrane-phase resistance is 2.7×106s/m，showing the resistance from the extraction reaction to be dominant.