A series of Schiff base containing dihydropyrimidinone (3a-3g) has been synthesized by condensation reactions of aromatic aldehydes with 4-(4-aminophenyl)-5-ethoxycarbonyl-6-methyl-3,4-dihydropyrimidone, which prepared from ethyl acetoacetate, 4-nitrobenzaldehyde and urea through condensation and reduction reactions. The compounds were characterized by IR、1H NMR and 13C NMR. The interactions between 3g and bovine serum albumin (BSA) were investigated using fluorescence spectroscopy at different temperatures, the results revealed that 3g can effectively quench the intrinsic fluorescence of BSA; the corresponding thermodynamic parameters shown that the action forces were mainly hydrophobic interaction; the binding distance was estimated to be about 2.59 nm according to F鰎ster’s non-radioactive energy transfer theory; the synchronous fluorescence showed that the 3g induced conformational changes of BSA