Abstract: Synthesis of L -2-methyl-tryptophan by immobilized cells of tryptophan synthetase cells was investigated. Using glutaraldehyde as crosslinking agent, sodium alginate as embedding agent, and modified corn straw stalk cellulose as filling agent immobilize the genetic engineering bacteria with tryptophan synthetase. Synthesis of L-2-methyl-tryptophan by immobilized cells of tryptophan synthetase engineering bacteria was carried out by response surface method. The effect of fumaric acid concentration, pH and reaction temperature was investigated. The optimized preparation conditions were as follows: 10 g/L L-serine, 35ºC, pH 8.0, the concentration of L-2-methyl-tryptophan was 5.3 g/L. The bioconversion rate of L-serine reached 41.6%. The yield of L-2-methyl-tryptophan was 25.3%. The immobilized bacteria could be used continuously for 12 times. The immobilized cells of tryptophan synthetase engineering bacteria by modified corn straw stalk cellulose have good stability.