The PDEGMA-b-POVNGA@AuNPs complex was prepared by a combination of enzymatic synthesis , reversible addition-fragment chain transfer (RAFT) polymerization protols and the “one-pot”method. The targeted glycomonomer 6-O-vinyladipoyl-α-D-galactose (OVNGA) and thermoresponsive diethyleneglycol methacrylate (DEGMA) as the monomers to form PDEGMA-b- POVNGA, the thermoresponsive glycopolymer. Then the PDEGMA-b-POVNGA combined with gold nanoparticals(AuNPs) to prepared PDEGMA-b-POVNGA@AuNPs complex by S—Au bond. The structure of PDEGMA-b-POVNGA was characterized by 1HNMR, FTIR and GPC. UV-visible spectroscopy tests showed that the low critical solution temperature (LSCT) of PDEGMA-b-POVNGA is about 35 ℃. Dynamic light scattering (DLS) demonstrated that the average diameter of AuNPs is 25nm. The PDEGMA-b-POVNGA@AuNPs complex was characterized by TEM. The result proved that the block polymer has grafted in the surface of AuNPs. UV-visible spectroscopy tests showed that the LSCT of PDEGMA-b-POVNGA@AuNPs is about 34.5 ℃. PDEGMA-b-POVNGA@AuNPs may have potential as functional drug carriers.