HA/CS/SiO2/Fe2O3 composite coating was prepared by electrophoretic deposition, and then HA/SiO2/Fe2O3 composite coating was obtained by sintering at 700 癈 for 2 h. The HA/SiO2/Fe2O3 composite coating was tested and characterized by FT-IR, SEM, EDS, XRD, Lloyd LR5K Plus Testing Instrument and Electrochemical Workstation, respectively. The antibacterial performance of HA/SiO2/Fe2O3 composite coating toward Bacillus Subtilis was investigated. The bioactivity of the HA/SiO2/Fe2O3 composite coating was evaluated by immersion in 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic-acid (HEPES) simulated body fluid (H-SBF). The results showed that the bonding strength between HA/SiO2/Fe2O3 composite coating and titanium substrate had reached 31.9 MPa, the surface of the composite coating had been completely carbonated hydroxyapatite after immersion in H-SBF for 14 days. The antibacterial test showed that the bacteriostasis rate of composite coating powder toward Bacillus Subtilis had been 81.9%. It is suitable for human beings bone implant material that the HA/SiO2/Fe2O3 composite coating possesses strong bonding strength and high biological activity and well antibacterial activity.