A series of ZnO, Al-ZnO and x-Mn-Al-ZnO adsorbents were synthesized by co-precipitation methods and investigated for the H2S desulfurization at ambient temperature.Thedifferencecaused by Mndopants in terms of structural properties, crystal structure, morphology and chemical state of reactive sites, were systematically investigated by N2adsorption, X-ray diffraction (XRD),scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS), respectively. The results show that with the increasing of Mn dopants, the desulfurization performance has two extreme points, when doping 0.02 mol and 0.08 molMn to Al-ZnO matrix. According to the characterization and desulfurization performance, different amount of Mn dopants leads to different promotion mechanism. The great performance of 0.02Mn-Al-ZnO is attributed to large surface area of 171.4 m2/g. Despite the surface area drops along with the increasing doping of Mn, more Mn enter into ZnO crystal lattice,which enhances the interaction between Zn and Mn, resulting in a promotion ofdesulfurization performance.However, the further doping ofMn results in smaller surface area and formation of ZnMn2O4, which causes poor desulfurization performance.And the highestsulfur capacity can be obtained over 0.08 Mn-Al-ZnO with 7.21g S/100 g sorbent, under 25oC, GHSV of 3000 h-1and 1000 ppmv H2S. It reveals clearly that doping difference amount of Mn can change morphology, crystal structure and physicochemical properties in varyingdegree, and each of them can act as a major factor leading to various desulfurization performance.