The flash extraction of total flavonoids from the leaves of Podocarpus macrophyllus has been optimized by response surface method, and antioxidant activities were studied. The design of extraction of total flavonoids is from Box-Behnken central composite experiment，at the same time, the scavenging activities on DPPH•、O2-•and •OH free radical of the total flavonoids of Podocarpus macrophyllus were measured. The results show that the extraction rate of total flavonoids of Podocarpus macrophyllus was 8.147%，and the optimum conditions were obtained as follows: the ethanol concentration was 71%，the material liquid ratio 1:36(g·mL-1)，the extraction time is 53s and the voltage is 75 V. Total flavonoids of Podocarpus macrophyllus have obvious scavenging ability to DPPH•、O2-•and •OH，and the maximum clearance rates were 87.13%, 58.24% and 67.56%，which can provide a foundation for the development of Podocarpus macrophyllus.